TFIIB-Termination Factor Interaction Affects Termination of Transcription on Genome-Wide Scale.

ABSTRACT: Apart from its well-established role in the initiation of transcription, the general transcription factor TFIIB has been implicated in the termination step as well. The ubiquity of TFIIB involvement in termination as well as mechanistic details of its termination function, however, remain largely unexplored. Using GRO-seq analyses, we compared the terminator readthrough phenotype in the sua7-1 mutant (TFIIB^sua7-1) and the isogenic wild type (TFIIB^WT) strains. Approximately 74% of genes analyzed exhibited a 2-3-fold increase in readthrough of the poly(A)-termination signal in the TFIIB^sua7-1 mutant compared to TFIIB^WT cells. To understand the mechanistic basis of TFIIB's role in termination, we performed the mass

SUBMITTER: O'Brien MJ 

PROVIDER: S-EPMC11354755 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature