Genetic Dissection of ToLCNDV Resistance in Resistant Sources of Cucumis melo.

ABSTRACT: Tomato leaf curl New Delhi virus (ToLCNDV) is a begomovirus causing significant melon (Cucumis melo) crop losses globally. This study aims to map the ToLCNDV resistance in the PI 414723 melon accession, previously identified and characterized through phenotypic studies, thereby exploring shared genomic regions with the established resistant source WM-7. In the present study, WM-7 and PI 414723 were crossed with the susceptible accessions 'Rochet' and 'Blanco' respectively, to generate F₁ hybrids. These hybrids were self-pollinated to generate the populations for mapping the ToLCNDV resistance region and designing markers for marker-assisted selection. Disease evaluation included visual symptom scoring, viral-load quantification and tissue printing. Genotyping-by-sequencin

SUBMITTER: Perez-Moro C 

PROVIDER: S-EPMC11354858 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature