Dataset Information

Magnaporthe-Unique Gene MUG1 Is Important for Fungal Appressorial Penetration, Invasive Hyphal Extension, and Virulence in Rice Blast Fungi.

ABSTRACT: Species-unique genes that encode specific proteins and have no homologs in other species play certain roles in the evolution of species and adaptations to external environments. Nevertheless, the biological roles of unique genes in plant pathogenic fungi remain largely unknown. Here, four Magnaporthe-unique genes (MUG1-MUG4), which were highly expressed during the early infection stages, were functionally characterized in the rice blast fungus Magnaporthe oryzae. Subcellular localization assays revealed that Mug1, Mug2, and Mug4 were localized to the cytoplasm and that Mug3 was localized into the nuclei. Furthermore, through gene knockout and phenotypic analysis, only MUG1 was found to be indispensable for fungal virulence and conidiation. Detailed microscopic analysis revealed that the deletion mutants of MUG1 clearly exhibited reduced appressorial turgor pressure and invasive hyphal development. Taken together, our findings indicate that the Magnaporthe-unique gene MUG1 plays a vital role in infection-related morphogenesis and virulence in rice blast fungi and suggest the specific and important roles of species-unique genes.

SUBMITTER: Zhang H

PROVIDER: S-EPMC11355306 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Magnaporthe-Unique Gene MUG1 Is Important for Fungal Appressorial Penetration, Invasive Hyphal Extension, and Virulence in Rice Blast Fungi.

Zhang Huixia H Chen Zhiyi Z Yu Zechen Z Tang Liu L Gao Wenqiang W Lu Xunli X Yang Jun J

Journal of fungi (Basel, Switzerland) 20240723 8

Species-unique genes that encode specific proteins and have no homologs in other species play certain roles in the evolution of species and adaptations to external environments. Nevertheless, the biological roles of unique genes in plant pathogenic fungi remain largely unknown. Here, four Magnaporthe-unique genes (MUG1-MUG4), which were highly expressed during the early infection stages, were functionally characterized in the rice blast fungus Magnaporthe oryzae. Subc ...[more]

PMID: 39194837

Similar Datasets

Project description:Endophytic fungi colonize plants without causing symptoms, throughout or at least a significant part of their life cycle, forming a plant-fungal association. In this study, endophytic fungi were isolated from Eucalyptus camaldulensis trees, and their antifungal activity was evaluated against four significant plant pathogens namely Botrytis cinerea, Fusarium oxysporum f. sp. lycopersici, Macrophomina phaseolina, and Rhizoctonia solani. For this aim, 754 fungal isolates were obtained from 44 healthy fruit, leaf, and branch samples collected from five provinces of Iran. Subsequently, 27 fungal genera were identified based on morphological characteristics and molecular data of ITS region, with Neofusicoccum, Cladosporium, Didymosphaeria, and Chaetomium being the most commonly found genera. Based on the morphological characteristics, 170 isolates were chosen and their antifungal activities were assessed against the aforementioned pathogens in vitro through dual culture and volatile organic compounds (VOCs) tests. Based on the results, five isolates comprising Trichoderma sp. KL1, Trichoderma sp. 8S1, Chaetomium sp. DL4, Phaeophleospora sp. XL4, and Pseudosydowia sp. VL3 were selected for further investigation, which included examining their chitinase and cellulase secretion capabilities as potential antagonism mechanisms and their ability to solubilize phosphate as a growth-promoting mechanism. Furthermore, the antifungal activity of the selected isolates was evaluated against plant pathogens on tomato plants under greenhouse conditions. Their impact on plant growth parameters was also assessed. In vitro and greenhouse experiments demonstrated that each selected isolate exhibited varying levels of control against different pathogens. Among the isolates, Trichoderma sp. isolates KL1 and 8S1 consistently exhibited the strongest inhibition of disease severity for all four pathogens under greenhouse conditions. Lastly, the selected isolates were identified as Trichoderma longibrachiatum KL1, T. longibrachiatum 8S1, Chaetomium globosum DL4, Phaeophleospora eucalypticola XL4, and Pseudosydowia eucalypti VL3 based on their morphological features and molecular data of the ITS1-5.8S-ITS2 and tef-1α genomic regions.

Dataset Information

Magnaporthe-Unique Gene MUG1 Is Important for Fungal Appressorial Penetration, Invasive Hyphal Extension, and Virulence in Rice Blast Fungi.

Publications

<i>Magnaporthe</i>-Unique Gene <i>MUG1</i> Is Important for Fungal Appressorial Penetration, Invasive Hyphal Extension, and Virulence in Rice Blast Fungi.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets