Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea.

ABSTRACT:

Background

A significant portion of South Korea's population, approximately a quarter, owns pets, with dogs being the most popular choice among them. However, studies analyzing the fecal organism communities of dogs in South Korea are lacking, and limited efforts have been exerted to identify pathogens with potential zoonotic implications. Therefore, this study aimed to investigate potential pathogens using metabarcoding analysis and evaluate the risk of zoonotic diseases in dog feces in Seoul, South Korea.

Methodology

Fecal samples were collected from both pet and stray dogs in the Mapo district of Seoul. Next-generation sequencing (NGS) was utilized, employing 16S rRNA amplicon sequencing to identify prokaryotic pathogens, and 18S rRNA amplicon sequencing for eukaryotic pa

SUBMITTER: Liyanagama I 

PROVIDER: S-EPMC11355564 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature