A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus neoformans.

ABSTRACT: The plethora of genome sequences produced in the postgenomic age has not resolved many of our most pressing biological questions. Correlating gene expression with an interrogatable and easily observable characteristic such as the surrogate phenotype conferred by a reporter gene is a valuable approach to gaining insight into gene function. Many reporters including lacZ, amdS, and the fluorescent proteins mRuby3 and mNeonGreen have been used across all manners of organisms. Described here is an investigation into the creation of a robust, synthetic, fusion reporter system for Cryptococcus neoformans that combines some of the most useful fluorophores available in this system with the versatility of the counter-selectable nature of amdS. The reporters generated incl

SUBMITTER: Phillips-Rose LS 

PROVIDER: S-EPMC11355724 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature