Methylated DNA Markers in Voided Urine for the Identification of Clinically Significant Prostate Cancer.

ABSTRACT:

Introduction

Non-invasive assays are needed to better discriminate patients with prostate cancer (PCa) to avoid over-treatment of indolent disease. We analyzed 14 methylated DNA markers (MDMs) from urine samples of patients with biopsy-proven PCa relative to healthy controls and further studied discrimination of clinically significant PCa (csPCa) from healthy controls and Gleason 6 cancers.

Methods

To evaluate the panel, urine from 24 healthy male volunteers with no clinical suspicion for PCa and 24 men with biopsy-confirmed disease across all Gleason scores was collected. Blinded to clinical status, DNA from the supernatant was analyzed for methylation signal within specific DNA sequences across 14 genes (HES5, ZNF655, ITPRIPL1, MAX.chr3.6187,

SUBMITTER: Shah P 

PROVIDER: S-EPMC11355912 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature