Fabrication of Porous Collagen Scaffolds Containing Embedded Channels with Collagen Membrane Linings.

ABSTRACT: Tissues and organs contain an extracellular matrix (ECM). In the case of blood vessels, endothelium cells are anchored to a specialized basement membrane (BM) embedded inside the interstitial matrix (IM). We introduce a multi-structural collagen-based scaffold with embedded microchannels that mimics in vivo structures within vessels. Our scaffold consists of two parts, each containing two collagen layers, i.e., a 3D porous collagen layer analogous to IM lined with a thin 2D collagen film resembling the BM. Enclosed microchannels were fabricated using contact microprinting. Microchannel test structures with different sizes ranging from 300 to 800 µm were examined for their fabrication reproducibility. The heights and perimeters of the fabricated microchannels were ~20% less than their corre

SUBMITTER: Fakhri N 

PROVIDER: S-EPMC11356104 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature