Evaluation of the Xpert Carba-R assay for quantifying carbapenemase-producing bacterial load in stool samples.

ABSTRACT:

Background

The spread of Carbapenemase-producing Organisms (CPO) remains a major threat globally. Within clinical settings, the existing method of determining gene load involves traditional culture to determine bacterial load and polymerase-chain-reaction-based Xpert Carba-R Assay to determine carbapenemase gene type. However, there is a need for a fast and accurate method of quantifying CPO colonisation to study the risk of persistent CPO carriage.

Objective

This study evaluated the accuracy of Xpert Carba-R Ct value in estimating carbapenamase producing bacterial loads in stool samples.

Methods

Stool samples were obtained from an ongoing study investigating the household transmission of CPO in Singapore. Stool samples lacking carbapenemase producing organisms were s

SUBMITTER: Chua JY 

PROVIDER: S-EPMC11356397 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature