A New Ex Vivo Model Based on Mouse Retinal Explants for the Study of Ocular Toxoplasmosis.

ABSTRACT: Ocular toxoplasmosis is the most prevalent clinical manifestation of T. gondii infection, which causes irreversible retinal damage. Different experimental models have been developed to study this pathology. In the present study, a new, ex vivo model is proposed to contribute to the elucidation of disease mechanisms and to possible therapeutic solutions. Ex-vivo retinal explants, prepared from mouse retinas following established protocols, were incubated with T. gondii tachyzoites maintained in Vero cells. At different times, starting at 12 h up to 10 days of incubation, the explants were analyzed with immunofluorescence and Western blot to investigate their responses to parasite infection. T. gondii invasion of the retinal thickness was evident after 3 days in culture,

SUBMITTER: Rodriguez Fernandez V 

PROVIDER: S-EPMC11356793 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature