Development of a Lateral Flow Assay for the Detection of the Hepatitis C Virus Core Antigen.

ABSTRACT:

Background

Hepatitis C virus (HCV) infection remains a global health challenge, with millions of people affected annually. Current diagnostic methods, reliant on antibody screening and viral RNA detection, are complex, costly, and often inaccessible, particularly in resource-limited settings.

Aim

Development of a lateral flow immunochromatography-based assay for detecting the highly conserved hepatitis C core antigen (HCVcAg).

Methods

The assay relies on the interaction of four highly specific and cross-reactive monoclonal antibodies with recombinant HCVcAg from five different genotypes in a double antibody sandwich format. Latex and colloidal gold were evaluated as detector nanoparticles.

Results

Extensive evaluation of 32 antibody combinations led to identify

SUBMITTER: Vidal-Alcantara EJ 

PROVIDER: S-EPMC11356972 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature