Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses.

ABSTRACT: Cell isolation protocols from brain tissue include prolonged ex vivo processing durations, rendering them suboptimal for transcriptomic studies. Particularly for microglia and vascular cells, current isolation methods produce lower yields, necessitating addition of an enrichment step, and use of large tissue volumes - in most cases whole brain tissue - to obtain sufficient yields. Here, we developed a simple, rapid, and reproducible cell isolation method for generating single-cell suspensions from micro-dissected brain regions, enriched for microglia and vascular cells, without an enrichment step. Cells isolated using this method are suitable for molecular profiling studies using 10 × Genomics Chromium single-cell RNA sequencing with high reproducibility. Our method is valuable for

SUBMITTER: Preka E 

PROVIDER: S-EPMC11357767 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature