Myotube formation on micropatterns guiding by centripetal cellular motility and crowding.

ABSTRACT: The physical microenvironment, including substrate rigidity and topology, impacts myoblast differentiation and myotube maturation. However, the interplay effect and physical mechanism of mechanical stimuli on myotube formation is poorly understood. In this study, we utilized elastic substrates, microcontact patterning technique, and particle image velocimetry to investigate the effect of substrate rigidity and topological constraints on myoblast behaviors. Our findings suggested the interplay of substrate stiffness and cellular confinement improved the myotube formation by inducing centripetal cellular motility. These results shed light on the impact of the topological substrate on myoblast differentiation and emphasize the critical role of asymmetrical cell motility during this process, w

SUBMITTER: Gao J 

PROVIDER: S-EPMC11357802 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature