FANCD2-FANCI surveys DNA and recognizes double- to single-stranded junctions.

ABSTRACT: DNA crosslinks block DNA replication and are repaired by the Fanconi anaemia pathway. The FANCD2-FANCI (D2-I) protein complex is central to this process as it initiates repair by coordinating DNA incisions around the lesion¹. However, D2-I is also known to have a more general role in DNA repair and in protecting stalled replication forks from unscheduled degradation^2-4. At present, it is unclear how DNA crosslinks are recognized and how D2-I functions in replication fork protection. Here, using single-molecule imaging, we show that D2-I is a sliding clamp that binds to and diffuses on double-stranded DNA. Notably, sliding D2-I stalls on encountering single-stranded-double-stranded (ss-ds) DNA junctions, structures that are generated when replication forks stall at DNA

SUBMITTER: Alcon P 

PROVIDER: S-EPMC11358013 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature