Validation of the C-X-C chemokine receptor 3 (CXCR3) as a target for PET imaging of T cell activation.

ABSTRACT:

Purpose

CXCR3 is expressed on activated T cells and plays a crucial role in T-cell recruitment to the tumor microenvironment (TME) during cell-based and immune checkpoint inhibitor (ICI) immunotherapy. This study utilized a ⁶⁴Cu-labeled NOTA-α-CXCR3 antibody to assess CXCR3 expression in the TME and validate it as a potential T cell activation biomarker in vivo.

Procedures

CXCR3⁺ cells infiltrating MC38 tumors (B57BL/6 mice, untreated and treated with αPD-1/αCTLA-4 ICI) were quantified using fluorescence microscopy and flow cytometry. A commercial anti-mouse CXCR3 antibody (α-CXCR3) was site-specifically conjugated with 2,2,2-(1,4,7-triazacyclononane-1,4,7-triyl)triacetic acid (NOTA) and radiolabeled with ⁶⁴Cu. Saturation binding of [^64<

SUBMITTER: Martin S 

PROVIDER: S-EPMC11358572 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature