Investigation of Extracted Plasma Cell-Free DNA as a Biomarker in Foals with Sepsis.

ABSTRACT: Cell-free DNA (cfDNA) is fragmented extracellular DNA that is released from cells into various body fluids. Previously published data from adult horses supports cfDNA as a potential disease biomarker, but also shows that direct measurement in plasma is inaccurate due to matrix effect. It is currently unknown whether a similar matrix effect exists in foal plasma. Given this, the objectives of the current study were to investigate foal plasma for potential matrix effect during fluorescence measurement of cfDNA using a Qubit fluorometer, and to determine whether neat and/or extracted plasma cfDNA concentrations are significantly different in healthy, sick non-septic (SNS) or septic foals. We hypothesized that matrix effect would interfere with accurate fluorescent measurement of cfDNA in foal

SUBMITTER: Hobbs KJ 

PROVIDER: S-EPMC11359113 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature