Immune Responses Induced by a Recombinant <i>Lactiplantibacillus plantarum</i> Surface-Displaying the gD Protein of Pseudorabies Virus.

ABSTRACT: Pseudorabies virus (PRV) is one of the herpes viruses that can infect a wide range of animals including pigs, cattle, sheep, mice, and wild animals. PRV is a neurotropic alphaherpesvirus capable of infecting a variety of mammals. There is a rising interest in the targeted application of probiotic bacteria to prevent viral diseases, including PRV. In this study, the surface expression of enhanced green fluorescent protein (EGFP) on recombinant Lactiplantibacillus plantarum NC8 (rNC8) through the LP3065 LPxTG motif of Lactobacillus plantarum WCFS1 was generated. The surface expression was observed through confocal microscopy. Dendritic cell targeting peptides (DCpep) were also fused with LPxTG that help to bind with mouse DCs. The PRV-gD was cloned in LP3065 LPxTG, resulting in

SUBMITTER: Moon A 

PROVIDER: S-EPMC11359135 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature