A Multimodal System for Lipid A Structural Analysis from a Single Colony.

ABSTRACT: Structural elucidation of Gram-negative bacterial lipid A traditionally requires chemical extraction followed by tandem MS data in the negative ion mode. Previously, we reported FLAT and FLATⁿ as methods to rapidly determine the structure of lipid A without chromatographic techniques. In this work, we extend the capability and effectiveness of these techniques to elucidate the chemical structure in a de novo manner by including the use of positive ion mode (FLAT⁺ and FLATⁿ⁺) spectral approaches. Advantages of positive mode analysis of lipid A include the generation of more interpretable and informative fragmentation patterns that include the identification of diagnostic fragments, including selective dissociation of a glycosidic bond between two glucosamine

SUBMITTER: Yang H 

PROVIDER: S-EPMC11359392 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature