Electrochemical Detection of Pseudomonas aeruginosa Quorum Sensing Molecule (S)-N-Butyryl Homoserine Lactone Using Molecularly Imprinted Polymers.

ABSTRACT: Pseudomonas aeruginosa is a multidrug-resistant Gram-negative bacterium that poses a significant threat to public health, necessitating rapid and on-site detection methods for rapid recognition. The goal of the project is therefore to indirectly detect the presence of P. aeruginosa in environmental water samples targeting one of its quorum-sensing molecules, namely, (S)-N-butyryl homoserine lactone (BHL). To this aim, molecularly imprinted polymers (MIPs) were synthesized via bulk free-radical polymerization using BHL as a template molecule. The obtained MIP particles were immobilized onto screen-printed electrodes (MIP-SPEs), and the BHL rebinding was analyzed via electrochemical impedance spectroscopy (EIS). To study the specificity of the synthesized MIPs, is

SUBMITTER: Frigoli M 

PROVIDER: S-EPMC11359617 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature