Identification of Dwarfing Candidate Genes in Brassica napus L. LSW2018 through BSA-Seq and Genetic Mapping.

ABSTRACT: Plant height, as a crucial component of plant architecture, exerts a significant influence on rapeseed (Brassica napus L.) lodging resistance, photosynthetic efficiency, yield, and mechanized harvest level. A previous study identified dwarf rapeseed LSW2018. In this study, LSW2018 (dwarf parent (PD)) was crossed with 389 (high parent (PH)) to establish the F₂ population, and 30 extremely dwarf (bulk-D) and high (bulk-H) plants in the F₂ population were respectively selected to construct two bulked DNA pools. Whole-genome sequencing and variation analysis (BSA-seq) were performed on these four DNA pools (PD, PH, bulk-D, and bulk-H). The BSA-seq results revealed that the genomic region responsible for the dwarf trait spanned from 19.30 to 22.19 Mb on chromosome A

SUBMITTER: Huang S 

PROVIDER: S-EPMC11359780 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature