Rapid discovery of death ligands with one-bead-two-compound combinatorial library methods.

ABSTRACT: The one-bead-one-compound (OBOC) technology enables one to generate thousands to millions of chemical molecules on resin beads (90 μm diameter) such that each bead displays 10(13) copies of the same chemical entity. Whole-cell binding assays have been developed to screen OBOC combinatorial libraries for ligands that bind to specific cell surface receptors. While very powerful, this screening method does not address the downstream cell signaling properties of the binding ligand. We have modified the OBOC technology by introducing a fixed known cell adhesion ligand to the outer layer of each bead. This one-bead-two-compound (OB2C) library configuration allows the bound cells to interact with the random immobilized chemical molecules on each bead. The bound cells can then be probed for specif

SUBMITTER: Kumaresan PR 

PROVIDER: S-EPMC11359996 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature