Nanofractionation Analytics for Comparing MALDI-MS and ESI-MS Data of <i>Viperidae</i> Snake Venom Toxins.

ABSTRACT: Worldwide, it is estimated that there are 1.8 to 2.7 million cases of envenoming caused by snakebites. Snake venom is a complex mixture of protein toxins, lipids, small molecules, and salts, with the proteins typically responsible for causing pathology in snakebite victims. For their chemical characterization and identification, analytical methods are required. Reversed-phase liquid chromatography coupled with electrospray ionization mass spectrometry (RP-LC-ESI-MS) is a widely used technique due to its ease of use, sensitivity, and ability to be directly coupled after LC separation. This method allows for the efficient separation of complex mixtures and sensitive detection of analytes. On the other hand, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is also some

SUBMITTER: Xu H 

PROVIDER: S-EPMC11360109 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature