Detection of multidrug-resistance in Mycobacterium tuberculosis by phenotype- and molecular-based assays.

ABSTRACT:

Background

The whole-genome sequencing (WGS) is becoming an increasingly effective tool for rapid and accurate detection of drug resistance in Mycobacterium tuberculosis complex (MTBC). This approach, however, has still been poorly evaluated on strains from Central and Eastern European countries. The purpose of this study was to assess the performance of WGS against conventional drug susceptibility testing (DST) for the detection of multi-drug resistant (MDR) phenotypes among MTBC clinical strains from Poland and Lithuania.

Methods

The study included 208 MTBC strains (130 MDR; 78 drug susceptible), recovered from as many tuberculosis patients in Lithuania and Poland between 2018 and 2021. Resistance to rifampicin (RIF) and isoniazid (INH) was assessed by Critical Concentrati

SUBMITTER: Vasiliauskaite L 

PROVIDER: S-EPMC11360294 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature