Identification of HPV16 Lineages in South African and Mozambican Women with Normal and Abnormal Cervical Cytology.

ABSTRACT:

Background

Human papillomavirus 16 (HPV16) is an oncogenic virus responsible for the majority of invasive cervical cancer cases worldwide. Due to genetic modifications, some variants are more oncogenic than others. We analysed the HPV16 phylogeny in HPV16-positive cervical Desoxyribonucleic Acid (DNA) samples collected from South African and Mozambican women to detect the circulating lineages.

Methods

Polymerase chain reaction (PCR) amplification of the long control region (LCR) and 300 nucleotides of the E6 region was performed using HPV16-specific primers on HPV16-positive cervical samples collected in women from South Africa and Mozambique. HPV16 sequences were obtained through Next Generation Sequencing (NGS) methods. Geneious prime and MEGA 11 software were used to alig

SUBMITTER: Maueia C 

PROVIDER: S-EPMC11360388 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature