Wound Healing in a Porcine Model of Retinal Holes.

ABSTRACT:

Purpose

To investigate retinal wound healing, we created a new porcine model of retinal hole and identified the cells involved in hole closure.

Methods

Sixteen landrace pigs underwent vitrectomy, and a subretinal bleb was created before cutting a retinal hole using a 23G vitrector. No tamponade was used. Before surgery and one, two, and four weeks after surgery, the eyes were examined by optical coherence tomography and color fundus photos. At the end of follow-up, the eyes were enucleated for histology. Tissue sections of 5 µm were prepared for hematoxylin-eosin staining and immunohistochemical analysis with antibodies to retinal glial and epithelial cells.

Results

Retinal holes below 1380 µm in diameter closed spontaneously within four weeks, whereas larger holes re

SUBMITTER: Olufsen ME 

PROVIDER: S-EPMC11361378 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature