A large-scale CRISPR screen reveals context-specific genetic regulation of retinal ganglion cell regeneration.

ABSTRACT: Many genes are known to regulate retinal regeneration after widespread tissue damage. Conversely, genes controlling regeneration after limited cell loss, as per degenerative diseases, are undefined. As stem/progenitor cell responses scale to injury levels, understanding how the extent and specificity of cell loss impact regenerative processes is important. Here, transgenic zebrafish enabling selective retinal ganglion cell (RGC) ablation were used to identify genes that regulate RGC regeneration. A single cell multiomics-informed screen of 100 genes identified seven knockouts that inhibited and 11 that promoted RGC regeneration. Surprisingly, 35 out of 36 genes known and/or implicated as being required for regeneration after widespread retinal damage were not required for RGC regeneration.

SUBMITTER: Emmerich K 

PROVIDER: S-EPMC11361637 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature