Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue.

ABSTRACT: Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth

SUBMITTER: Seo EJ 

PROVIDER: S-EPMC11361853 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature