Expression of IL-1β in transgenic <i>Eimeria necatrix</i> enhances the immunogenicity of parasites and promotes mucosal immunity against coccidiosis.

ABSTRACT: Anticoccidial vaccines comprising living oocysts of Eimeria tenella, Eimeria necatrix, Eimeria maxima, and Eimeria acervulina are used to control coccidiosis. This study explored the potential of IL-1β to act as a molecular adjuvant for enhancing the immunogenicity of Eimeria necatrix and mucosal immunity. We engineered E. necatrix to express a functional chIL-1β (EnIL-1β) and immunized chickens with oocysts of the wild type (EnWT) and tranegenic (EnIL-1β) strains, respectively. The chickens were then challenged with EnWT oocysts to examine the immunogenicity-enhancing potential of chIL-1β. As expected, the oocyst output of EnIL-1β-immunized chickens was significantly reduced compared to those immunized using EnWT. No difference in body weight gain and lesion sc

SUBMITTER: Duan C 

PROVIDER: S-EPMC11361970 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature