Protein assemblies in the Arabidopsis thaliana chloroplast compartment.

ABSTRACT:

Introduction

Equipped with a photosynthetic apparatus that uses the energy of solar radiation to fuel biosynthesis of organic compounds, chloroplasts are the metabolic factories of mature leaf cells. The first steps of energy conversion are catalyzed by a collection of protein complexes, which can dynamically interact with each other for optimizing metabolic efficiency under changing environmental conditions.

Materials and methods

For a deeper insight into the organization of protein assemblies and their roles in chloroplast adaption to changing environmental conditions, an improved complexome profiling protocol employing a MS-cleavable cross-linker is used to stabilize labile protein assemblies during the organelle isolation procedure.

Results and discussion

Changes

SUBMITTER: Ditz N 

PROVIDER: S-EPMC11362043 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature