In-vitro study on type I collagen synthesis in low-level laser therapy on the early ligament fibroblasts' healing process.

ABSTRACT:

Background

Low-level Laser Therapy (LLLT) has demonstrated its potential in promoting fiber matrix maturation, collagen synthesis, and fibroblast proliferation, contributing to tissue regeneration. Our study aimed to investigate the impact of LLLT on collagen type I synthesis, cell proliferation, and viability in human ligament fibroblasts derived from the Anterior Cruciate Ligament (ACL).

Methods

Tissue samples were obtained from individuals undergoing arthroscopic ACL reconstruction surgery. Primary human fibroblasts were isolated, and immunohistochemical assays confirmed their characteristics. LLLT at 850 nm was administered in three groups: Low dose (1.0 J/cm²), High dose (5.0 J/cm²), and Control (0.0 J/cm²). Cell viability was calculated using a membrane integrity assay

SUBMITTER: Cardenas-Sandoval RP 

PROVIDER: S-EPMC11362177 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature