Elevated level of extracellular vimentin is associated with an increased fibrin formation potential in sepsis: ex vivo swine study.

ABSTRACT:

Background

Sepsis can lead to coagulopathy and microvascular thrombosis. Prior studies, including ours, reported an increased level of extracellular vimentin in blood derived from septic patients. Moreover, we identified the contribution of extracellular vimentin to fibrin formation and to the fibrin clot structure ex vivo in plasma from septic patients. Here, we tested the status of plasma vimentin and its impact on fibrin clots using our recently described swine model of methicillin-resistant Staphylococcus aureus (MRSA) sepsis-induced coagulopathy.

Results

We employed ELISA, size-exclusion chromatography, vimentin antibodies, confocal microscopy, and turbidity assays on piglet plasma obtained at pre- and post-MRSA inoculation. Plasma vimentin level at 70 h post-MRSA inocu

SUBMITTER: Martinez-Vargas M 

PROVIDER: S-EPMC11362409 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature