Chemigenetic Far-Red Labels and Ca²⁺ Indicators Optimized for Photoacoustic Imaging.

ABSTRACT: Photoacoustic imaging is an emerging modality with significant promise for biomedical applications such as neuroimaging, owing to its capability to capture large fields of view deep inside complex scattering tissue. However, widespread adoption of this technique has been hindered by a lack of suitable molecular reporters for this modality. In this work, we introduce chemigenetic labels and calcium sensors specifically tailored for photoacoustic imaging, using a combination of synthetic dyes and HaloTag-based self-labeling proteins. We rationally design and engineer far-red "acoustogenic" dyes, showing high photoacoustic turn-ons upon binding to HaloTag, and develop a suite of tunable calcium indicators based on these scaffolds. These first-generation photoacoustic reporters show excellent

SUBMITTER: Cook A 

PROVIDER: S-EPMC11363013 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature