Conserved 5-methyluridine tRNA modification modulates ribosome translocation.

ABSTRACT: While the centrality of posttranscriptional modifications to RNA biology has long been acknowledged, the function of the vast majority of modified sites remains to be discovered. Illustrative of this, there is not yet a discrete biological role assigned for one of the most highly conserved modifications, 5-methyluridine at position 54 in tRNAs (m⁵U54). Here, we uncover contributions of m⁵U54 to both tRNA maturation and protein synthesis. Our mass spectrometry analyses demonstrate that cells lacking the enzyme that installs m⁵U in the T-loop (TrmA in Escherichia coli, Trm2 in Saccharomyces cerevisiae) exhibit altered tRNA modification patterns. Furthermore, m⁵U54-deficient tRNAs are desensitized to small molecules that prevent transl

SUBMITTER: Jones JD 

PROVIDER: S-EPMC11363252 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature