Chemical Synthesis of Human Proteoforms and Application in Biomedicine.

ABSTRACT: Limited understanding of human proteoforms with complex posttranslational modifications and the underlying mechanisms poses a major obstacle to research on human health and disease. This Outlook discusses opportunities and challenges of de novo chemical protein synthesis in human proteoform studies. Our analysis suggests that to develop a comprehensive, robust, and cost-effective methodology for chemical synthesis of various human proteoforms, new chemistries of the following types need to be developed: (1) easy-to-use peptide ligation chemistries allowing more efficient de novo synthesis of protein structural domains, (2) robust temporary structural support strategies for ligation and folding of challenging targets, and (3) efficient transpeptidative protein domain-domain li

SUBMITTER: Ai H 

PROVIDER: S-EPMC11363345 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature