GFPT1 accelerates immune escape in breast cancer by modifying PD-L1 via O-glycosylation.

ABSTRACT:

Background

Immune escape is one of the causes of poor prognosis in breast cancer (BC). Glutamine-fructose-6-phosphate transaminase 1 (GFPT1) is the first speed-limiting enzyme of the hexosamine biosynthesis pathway (HBP) and is essential for the progression of BC. Nevertheless, the mechanism of the influence of GFPT1 in BC immune escape is not clear.

Methods

First, the level of GFPT1 in BC was analyzed by starbase, and GFPT1 expression in BC tissues was measured by qRT-PCR, western blot and IHC. Then, the O-GlcNAc levels were detected by western blot. Thereafter, Co-IP was applied to examine the relationship between GFPT1 and PD-L1. At last, a mouse model was constructed for validation in vivo.

Results

Firstly, we discovered that GFPT1 was obviously strengthened in BC

SUBMITTER: Tang W 

PROVIDER: S-EPMC11363670 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature