Real-time assessment of relative mitochondrial ATP synthesis response against inhibiting and stimulating substrates (MitoRAISE).

ABSTRACT:

Background

Mitochondria are known to synthesize adenosine triphosphate (ATP) through oxidative phosphorylation. Understanding and accurately measuring mitochondrial ATP synthesis rate can provide insights into the functional status of mitochondria and how it contributes to overall cellular energy homeostasis. Traditional methods only estimate mitochondrial function by measuring ATP levels at a single point in time or through oxygen consumption rates. This study introduced the relative mitochondrial ATP synthesis response against inhibiting and stimulating substrates (MitoRAISE), designed to detect real-time changes in ATP levels as the cells respond to substrates.

Methods

The sensitivity and specificity of the MitoRAISE assay were verified under various conditions, including

SUBMITTER: Chang ES 

PROVIDER: S-EPMC11363686 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature