Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics.

ABSTRACT: Allergic contact dermatitis (ACD), a prevalent inflammatory skin disease, is elicited upon repeated skin contact with protein-reactive chemicals through a complex and poorly characterized cellular network between immune cells and skin resident cells. Here, single-cell transcriptomic analysis of the murine hapten-elicited model of ACD reveals that upon elicitation of ACD, infiltrated CD4⁺ or CD8⁺ lymphocytes were primarily the IFNγ-producing type 1 central memory phenotype. In contrast, type 2 cytokines (IL4 and IL13) were dominantly expressed by basophils, IL17A was primarily expressed by δγ T cells, and IL1β was identified as the primary cytokine expressed by activated neutrophils/monocytes and macrophages. Furthermore, analysis of skin resident cells identified a su

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11364439 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature