Cell sorting based on pulse shapes from angle resolved detection of scattered light.

ABSTRACT: Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest. However, such stains also have disadvantages, as their effect on cells must be carefully tested to avoid effects on the results of the experiments. Alternative approaches using imaging or other label-free techniques often require highly sophisticated setups, are commonly limited in resolution, and produce challenging amounts of data. Our technology exploits scattered light instead. The custom-built flow cytometry setup comprises a fiber array in forward scatter detection for angular resolution and captures the whole pulse shape with advanced signal processing. Thereby this

SUBMITTER: Kage D 

PROVIDER: S-EPMC11364749 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature