Integrated single-cell and bulk RNA-seq analysis identifies a prognostic T-cell signature in colorectal cancer.

ABSTRACT: Colorectal cancer (CRC) is a major contributor to global morbidity and mortality, necessitating more effective therapeutic approaches. T cells, prominent in the tumor microenvironment, exert a crucial role in modulating immunotherapeutic responses and clinical outcomes in CRC. This study introduces a pioneering method for characterizing the CRC immune microenvironment using single-cell sequencing data. Unlike previous approaches, which focused on individual T-cell signature genes, we utilized overall infiltration levels of colorectal cancer signature T-cells. Through weighted gene co-expression network analysis, Lasso regression, and StepCox analysis, we developed a prognostic risk model, TRGS (T-cell related genes signatures), based on six T cell-related genes. Multivariate Cox analysis i

SUBMITTER: Cui P 

PROVIDER: S-EPMC11364821 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature