Label-free spatiotemporal decoding of single-cell fate via acoustic driven 3D tomography.

ABSTRACT: Label-free three-dimensional imaging plays a crucial role in unraveling the complexities of cellular functions and interactions in biomedical research. Conventional single-cell optical tomography techniques offer affordability and the convenience of bypassing laborious cell labelling protocols. However, these methods are encumbered by restricted illumination scanning ranges on abaxial plane, resulting in the loss of intricate cellular imaging details. The ability to fully control cellular rotation across all angles has emerged as an optimal solution for capturing comprehensive structural details of cells. Here, we introduce a label-free, cost-effective, and readily fabricated contactless acoustic-induced vibration system, specifically designed to enable multi-degree-of-freedom rotation of

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11364901 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature