Silk films with distinct surface topography modulate plasma membrane curvature to polarize macrophages.

ABSTRACT: The physical properties of a biomaterial play a vital role in modulating macrophage polarization. However, discerning the specific effects of individual parameters can be intricate due to their interdependencies, limiting the mechanism underlying a specific parameter on the polarization of macrophages. Here, we engineered silk fibroin (SF) films with tunable surface roughness while maintaining similar physical properties by combining casting and salting out techniques. We demonstrate that increased surface roughness in SF films promotes M2-like macrophage polarization, characterized by enhanced secretion of anti-inflammatory cytokines. Transcriptomic analysis unveils the modulation of genes associated with extracellular matrix-cell interactions, highlighting the role of surface topography

SUBMITTER: Hu D 

PROVIDER: S-EPMC11364906 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature