Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii.

ABSTRACT: Komagataella phaffii (Pichia pastoris) is a methylotrophic yeast that is favored by industry and academia mainly for expression of heterologous proteins. However, its full potential as a host for bioproduction of valuable compounds cannot be fully exploited as genetic tools are lagging behind those that are available for baker's yeast. The emergence of CRISPR-Cas9 technology has significantly improved the efficiency of gene manipulations of K. phaffii, but improvements in gene-editing methods are desirable to further accelerate engineering of this yeast. In this study, we have developed a versatile vector-based CRISPR-Cas9 method and showed that it works efficiently at different genetic loci using linear DNA fragments with very short targeting sequences including single-stranded oligonucle

SUBMITTER: Strucko T 

PROVIDER: S-EPMC11364938 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature