Identifying the molecular basis of Laminin N-terminal domain Ca²⁺ binding using a hybrid approach.

ABSTRACT: Ca²⁺ is a highly abundant ion involved in numerous biological processes, particularly in multicellular eukaryotic organisms where it exerts many of these functions through interactions with Ca²⁺ binding proteins. The laminin N-terminal (LN) domain is found in members of the laminin and netrin protein families where it plays a critical role in the function of these proteins. The LN domain of laminins and netrins is a Ca²⁺ binding domain and in many cases requires Ca²⁺ to perform its biological function. Here, we conduct a detailed examination of the molecular basis of the LN domain Ca²⁺ interaction combining structural, computational, bioinformatics, and biophysical techniques. By combining computational and bioinformatic techniques wit

SUBMITTER: Legare S 

PROVIDER: S-EPMC11365105 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature