Coarse-grained modeling of annexin A2-induced microdomain formation on a vesicle.

ABSTRACT: Annexin A2 (A2)-induced microdomain formation is a key step in biological processes such as Ca²⁺-mediated exocytosis in neuroendocrine cells. In this work, a total of 15 coarse-grained molecular dynamics simulations were performed on vesicle models having a diameter of approximately 250 Å for 15 μs each using the Martini2 force field. Five simulations were performed in the presence of 10 A2, 5 in the presence of A2 but absence of PIP₂, and 5 simulations in the absence of A2 but presence of PIP₂. Consistent results were generated among the simulations. A2-induced PIP₂ microdomain formation was observed and shown to occur in three phases: A2-vesicle association, localized A2-induced PIP₂ clustering, and A2 aggregation driving PIP_2</

SUBMITTER: Lindsay S 

PROVIDER: S-EPMC11365106 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature