The molecular basis for hydrodynamic properties of PEGylated human serum albumin.

ABSTRACT: Polyethylene glycol (PEG) conjugation provides a protective modification that enhances the pharmacokinetics and solubility of proteins for therapeutic use. A knowledge of the structural ensemble of these PEGylated proteins is necessary to understand the molecular details that contribute to their hydrodynamic and colligative properties. Because of the large size and dynamic flexibility of pharmaceutically important PEGylated proteins, the determination of structure is challenging. In addition, the hydration of these conjugates that contain large polymers is difficult to determine with traditional methods that identify only first shell hydration water, which does not account for the complete hydrodynamic volume of a macromolecule. Here, we demonstrate that structural ensembles, generated by

SUBMITTER: Fleming PJ 

PROVIDER: S-EPMC11365107 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature