Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.

ABSTRACT: The asymmetry of membranes has a significant impact on their biophysical characteristics and behavior. This study investigates the composition and mechanical properties of symmetric and asymmetric membranes in giant unilamellar vesicles (GUVs) made of palmitoyloleoyl phosphatidylcholine (POPC) and palmitoyloleoyl phosphatidic acid (POPA). A combination of fluorescence quantification, zeta potential measurements, micropipette aspiration, and bilayer molecular dynamics simulations are used to characterize these membranes. The outer leaflet composition in vesicles is found consistent across the two preparation methods we employed, namely electroformation and inverted emulsion transfer. However, characterizing the inner leaflet poses challenges. Micropipette aspiration of GUVs show that oil re

SUBMITTER: Drabik D 

PROVIDER: S-EPMC11365108 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature