Impact of internal standard selection on measurement results for long chain fatty acids in blood.

ABSTRACT:

Introduction

Internal standards correct for measurement variation due to sample loss. Isotope labeled analytes are ideal internal standards for the measurement of fatty acids in human plasma but are not always readily available. For this reason, quantification of multiple analytes at once is most often done using only a single or few internal standards. The magnitude of the impact this has on method accuracy and precision is not well studied for gas chromatography-mass spectrometry systems.

Objective

This study aims to estimate bias and changes in uncertainty associated with using alternative fatty acid isotopologue internal standards for the estimation of similar or dissimilar long chain fatty acids.

Method

Using a previously reported method for the quantification of

SUBMITTER: Goodwin JM 

PROVIDER: S-EPMC11365363 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature