Biolayer interferometry for adeno-associated virus capsid titer measurement and applications to upstream and downstream process development.

ABSTRACT: Faster and more accurate analytical methods are needed to support the advancement of recombinant adeno-associated virus (rAAV) production systems. Recently, biolayer interferometry (BLI) has been developed for high-throughput AAV capsid titer measurement by functionalizing the AAVX ligand onto biosensor probes (AAVX-BLI). In this work, an AAVX-BLI method was evaluated using Octet AAVX biosensors across four rAAV serotypes (rAAV2, -5, -8, and -9) and applied in an upstream and downstream processing context. AAVX-BLI measured the capsid titer across a wide concentration range (1 × 10¹⁰-1 × 10¹² capsids/mL) for different rAAV serotypes and sample backgrounds with reduced measurement variance and error compared to an enzyme-linked immunosorbent assay (ELISA) method. Biose

SUBMITTER: Leibiger TM 

PROVIDER: S-EPMC11365433 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature