Targeted isolation of Methanobrevibacter strains from fecal samples expands the cultivated human archaeome.

ABSTRACT: Archaea are vital components of the human microbiome, yet their study within the gastrointestinal tract (GIT) is limited by the scarcity of cultured representatives. Our study presents a method for the targeted enrichment and isolation of methanogenic archaea from human fecal samples. The procedure combines methane breath testing, in silico metabolic modeling, media optimization, FACS, dilution series, and genomic sequencing through Nanopore technology. Additional analyzes include the co-cultured bacteriome, comparative genomics of archaeal genomes, functional comparisons, and structure-based protein function prediction of unknown differential traits. Successful establishment of stable archaeal cultures from 14 out of 16 fecal samples yielded nine previously uncultivated strains, eight of

SUBMITTER: Duller S 

PROVIDER: S-EPMC11366006 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature