Alternation of gene expression in brain-derived exosomes after cerebral ischemic preconditioning in mice.

ABSTRACT:

Aims

Cerebral ischemic preconditioning is a neuroprotective therapy against cerebral ischemia and ischemia-reperfusion injury. This study aims to demonstrate the alternation of gene expression in exosomes from brain tissue of mice after ischemic preconditioning and their potential functions.

Methods

Ten mice were divided into the sham and the cerebral ischemic preconditioning groups. Their brain tissues were harvested, from which the exosomes were extracted. The characteristics and protective effects of exosomes were evaluated. Whole transcriptome sequencing was used to demonstrate the gene expression discrepancy between the exosomes from the two groups of mice brains. Volcano graphs and heatmaps were used to picture the difference in expression quantity of mRNA, lncRNA, and

SUBMITTER: Li H 

PROVIDER: S-EPMC11367060 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature