Novel insights into the modulation of the voltage-gated potassium channel K_V1.3 activation gating by membrane ceramides.

ABSTRACT: Membrane lipids extensively modulate the activation gating of voltage-gated potassium channels (K_V), however, much less is known about the mechanisms of ceramide and glucosylceramide actions including which structural element is the main intramolecular target and whether there is any contribution of indirect, membrane biophysics-related mechanisms to their actions. We used two-electrode voltage-clamp fluorometry capable of recording currents and fluorescence signals to simultaneously monitor movements of the pore domain (PD) and the voltage sensor domain (VSD) of the K_V1.3 ion channel after attaching an MTS-TAMRA fluorophore to a cysteine introduced into the extracellular S3-S4 loop of the VSD. We observed rightward shifts in the conductance-voltage (G-V) relationship

SUBMITTER: Cs Szabo B 

PROVIDER: S-EPMC11367112 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature